restriction enzymes bam hi (TaKaRa)

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TaKaRa restriction enzymes bam hi
Restriction Enzymes Bam Hi, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 14709 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/restriction+enzymes+bam+hi/BamH+I/pm40723886-78-7-14
Average 99 stars, based on 14709 article reviews

restriction enzymes bam hi - by Bioz Stars, 2026-10

99/100 stars

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Sequencing:

Article Title: Aberrant expression of the extracellular matrix component Biglycan regulated by Hedgehog signalling promotes colorectal cancer cell proliferation
Article Snippet: .. Specifically, the coding sequence of BGN was amplified with the indicated primers, digested with the restriction enzymes Bam HI and Xho I, and inserted into the empty vector pLVX (Clontech, Mountain View, USA). .. The BGN promoter was amplified from HCT116 genomic DNA via PCR with primers, cut with the restriction enzymes Nhe I and Eco RV, and inserted into the empty vector pGL4.2 (Promega, Madison, USA).

Amplification:

Article Title: Interspecies Recombination-Led Speciation of a Novel Geminivirus in Pakistan
Article Snippet: Total nucleic acid was extracted from the samples using the Viral Gene-spin Viral DNA/RNA Extraction Kit (iNtRON Biotechnology, Inc., Seongnam, Korea) following the manufacturer’s instructions. .. Circular DNA was amplified using the extracted total DNA as a template through rolling circle amplification (RCA) (TempliPhi Amplification Kit; GE Healthcare Life Sciences, Uppsala, Sweden) before being digested with the restriction enzymes Bam HI, Xho I, Hind III, and Eco RV (TaKaRa Bio, Inc., Shiga, Japan) [ , ]. ..

other:

Article Title: Characterization of the YCjN ABC Transporter in Escherichia coli : Role in Maltose and Ethidium Bromide Transport
Article Snippet: Restriction enzymes Bam HI, Hind III, and Dpn I (TaKaRa, Beijing, China).

Article Title: Characterization of the YCjN ABC Transporter in Escherichia coli: Role in Maltose and Ethidium Bromide Transport.
Article Snippet: This study investigated the functional role of the ycjN gene in Escherichia coli, hypothesizing that its encoded protein, YcjN, functions as a carbohydrate transporter.. Bioinformatics analysis confirmed YcjN as a solute-binding protein (SBP).. The construction of a ΔycjN deletion strain revealed that ycjN is not essential for bacterial viability.

Plasmid Preparation:

Article Title: Production of acetoin and its derivative tetramethylpyrazine from okara hydrolysate with Bacillus subtilis
Article Snippet: The arabinose transporter-encoding gene araE and galactose-related gene cluster galKTE (Gene ID:945358,945357,945354) were amplified from the genomes of B. subtilis 168 and E. coli MG1655, respectively, using 2 × Phanta Max Master Mix DNA polymerase (Vazyme, Nanjing, China). .. The pMA5 plasmid was digested with restriction enzymes Bam HI and MIu I (Takara, Beijing, China), and each gene was ligated into the vector using the ClonExpress Ultra One Step Cloning Kit (Vazyme, Nanjing, China). ..

Cloning:

Polymerase Chain Reaction:

Article Title: The Superoxide Dismutase Gene Family in Nicotiana tabacum : Genome-Wide Identification, Characterization, Expression Profiling and Functional Analysis in Response to Heavy Metal Stress
Article Snippet: .. The PCR fragment and pYES2 vector were digested using the restriction enzymes Bam HI and Eco RI (TaKaRa, Dalian, China) for 3 h at 37°C. ..

Expressing:

Article Title: A Type Ia Crustin from the Pacific White Shrimp Litopenaeus vannamei Exhibits Antimicrobial and Chemotactic Activities
Article Snippet: .. The expression vector pET28a was linearized using restriction enzymes Bam HI and Hind III (TakaRa, Dalian, China). .. Subsequently, the purified PCR fragment was ligated into the digested vector with the In-Fusion HD Cloning Kit (TakaRa, Dalian, China).

Article Title: A Type Ia Crustin from the Pacific White Shrimp Litopenaeus vannamei Exhibits Antimicrobial and Chemotactic Activities.
Article Snippet: .. The expression vector pET28a was linearized using restriction enzymes Bam HI and Hind III (TakaRa, Dalian, China). .. Subsequently, the purified PCR fragment was ligated into the digested vector with the In-Fusion HD Cloning Kit (TakaRa, Dalian, China).

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